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To observe different expression patterns of β-catenin and its clinical significance in colorectal cancer (CRC).Methods A total of 181 cases of CRC tissues and 30 cases of normal colorectal tissue were investigated by immunohistochemistry for the expression of β-catenin.Results The expression rate of β-catenin was 56.9％ (103/181) in CRC,and higher than that in normal colorectal tissue (P ＜ 0.05).The overexpression of nuclear β-catenin was significantly correlated with histological differentiation,lymph node metastasis and Dukes' stage in CRC (P ＜ 0.05),and no relationship with other pathological parameters,such as age,gender and the depth of infiltration.The incomplete membranous expression of β-catenin was significantly correlated with histological differentiation,the depth of infiltration,lymph node metastasis and Dukes' stage in CRC (P ＜ 0.05).The high expression of nuclear β-catenin related to histological differentiation and Dukes' stage in CRC (P ＜ 0.05).In the follow-up data of 82 cases of CRC,the expression of nuclear β-catenin was associated with poor prognosis,and the 5-year survival rate was significantly lower than that of self-control groups (P ＜ 0.05).Conclusion β-catenin plays important roles in colorectal carcinogenesis.Abnormal expression of β-catenin was related to the aggressive progression of CRC and may be helpful for evaluating the prognosis of patients with CRC.β-catenin is expected to become a new target for diagnosis and treatment of CRC in future.
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Objective To investigate the expression of FZD7 (frizzled homolog protein7,FZD7) and IL-6 (interleukin-6,IL-6) in primary hepatocellular carcinoma(HCC) and the impact on the prognosis.Methods Immunohistochemistry(IHC) was used to detect the expression level of FZD7 and IL-6 in 68 HCC tissues,30 para-carcinoma tissues and 10 healthy controls from patients with hepatic hemangioma.Patients were followed-up for survival analysis.Results The expression ratio of FZD7 in HCC tissue,paracarcinoma tissue and healthy controls were 72％,53％ and 0 (P ＜ 0.001),the ratio of IL-6 expression was respectively 69％,57％ and 0 (P ＜0.001).The expressions of FZD7 were positively correlated with that of AFP level (x2 =7.059,P =0.035),pathology classification (x2 =4.732,P =0.043),and the infection of HBs (x2 =3.508,P =0.032).The overall survival (65 ％ vs.84％,x2 =5.525,P ＜ 0.05) and the disease free survival (33％ vs.75％,x2 =5.991,P ＜0.05) of HCC patients with co-positive was significantly lower than that with co-negative expression of FZD7 and IL-6.Conclusion FZD7 and IL-6 play a critical role in development and progression of HCC.
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Purpose To investigate the expression and clinical significance of microRNA-21 (miR-21) in patients with prostate cancer (PCa).Methods The human tissue specimens were paraffin-embedded blocks which were collected from the Department of Pathology.miR-21 was detected by in situ hybridization (ISH) in 90 cases of PCa and 69 cases of benign prostatic hyperplasia (BPH).Results The ISH data showed that the positive rate of miR-21 in PCa (71.1％) was significantly higher than that in BPH (24.6％) (P ＜ 0.05),the expression of miR-21 correlated with the Gleason grade (P ＜ 0.05),and no relationship was observed with other clinicpathological features (P ＞ 0.05).Conclusion The miR-21 plays an important role in the pathogenesis of PCa,which can be a new diagnostic target for PCa.
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<p><b>OBJECTIVE</b>To study the role of microRNAs (miRNAs) in ALK-negative anaplastic large cell lymphoma and CD30 positive peripheral T cell lymphoma (not otherwise specified), and discuss the pathogenesis of miRNAs in ALK-negative anaplastic large cell lymphoma.</p><p><b>METHODS</b>Three cases of ALK-negative anaplastic large cell lymphoma of lymph node, 3 cases of CD30-positive peripheral T cell lymphoma (not otherwise specified) of lymph node and 3 cases of reactive hyperplasia of lymph node were detected by high flow microarray of miRNAs. The method of real-time quantitative polymerase chain reaction was further applied for 7 miRNAs in 15 cases of ALK-negatie anaplastic large cell lymphomas of lymph node and 15 cases of CD30-positive peripheral T cell lymphoma (not otherwise specified) of lymph node.</p><p><b>RESULTS</b>The significant difference of 13 miRNAs was found between ALK-negative anaplastic large cell lymphoma and CD30 positive peripheral T cell lymphoma (not otherwise specified) (P < 0.05), of which the result of 5 miRNAs was consistent with miRNAs expression spectrum: miR-664b-5p, miR-1275, miR-4739, miR-4736 and miR-504-5p, the difference was statistically significant (P < 0.05). Compared with reactive hyperplasia of lymph nodes, miR-664b-5p, miR-1275 and miR-4739 were significantly under-expressed (P = 0.004, P = 0.021, P = 0.031) and miR-4736 and miR-504-5p were significantly over-expressed (P = 0.009, P = 0.007) in ALK negative anaplastic large cell lymphoma.</p><p><b>CONCLUSIONS</b>MiR-664b-5p, miR-1275, miR-4739, miR-4736 and miR-504-5p may become an important indicator in the differentiation ALK-negative anaplastic large cell lymphoma from CD30-positive peripheral T cell lymphoma (not otherwise specified). MiR-4739, miR-4736 and miR-1275 may play important role in pathogenesis of negative-anaplastic large cell lymphoma by target genes: TNFRSF8 and TMOD1.</p>
Subject(s)
Humans , Ki-1 Antigen , Metabolism , Lymphoma, Large-Cell, Anaplastic , Diagnosis , Metabolism , Lymphoma, T-Cell, Peripheral , Diagnosis , Metabolism , MicroRNAs , Metabolism , Real-Time Polymerase Chain ReactionABSTRACT
Purpose To investigate the clinicopathological features and prognosis in patients with colorectal cribriform comedo-type ad-enocarcinoma. Methods Compared with 278 cases of colorectal common-type adenocarcinoma, 24 cases of colorectal cribriform come-do-type adenocarcinoma were analyzed for clinical stage, lymphovascular invasion, lymph node metastasis and genetic characteristics. Results The percentage of clinical Ⅲ and Ⅳ (87. 5%) and lymph node metastasis rate (87. 5%) in patients with colorectal cribri-form comedo-type adenocarcinoma was obviously higher than that in colorectal common-type adenocarcinoma (42. 4%,42. 4%) (P0. 05 ) . Conclusions There is a higher clinical stage and poorer prognosis including unique morphological characteristics, highly lymphovascular invasion and lymph node metastasis in pa-tients with colorectal cribriform comedo-type adenocarcinoma.
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Purpose To investigate the expression and clinical significance of p63 in nodular lymphocyte predominant Hodgkin lym-phoma (NLPHL) and classical Hodgkin lymphoma (CHL). Methods 15 cases of NLPHL and 54 cases of CHL were stained for CD45, CD30, p63, PAX5, CD20, CD15, Oct-2, BOB1, MUM1, EMA, EBV-LMP1 and Ki-67 by immunohistochemical methods of EliVision. EBER were detected by in situ hybridization method in 12 cases of CHL. Results The expression of p63 in NLPHL (53. 3%, 8/15) was significantly higher than that in CHL (0, 0/54) (P<0. 05). Conclusions p63 protein is frequently expressed in NLPHL and helpful in the differential diagnosis between NLPHL and CHL.
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Objective To investigate the roles of infection of Helicobacter priori (Hp) and effects of Hp eradication in the development of gastric carcinoids in Mongolian gerbils. Methods A total of 100 an-imals were divided into seven groups: A, B without Hp were sacrificed after 50 and 100 weeks as controls, Hp was inoculated into group C, D, E, F, G. Group F and G received eradication of Hp after 75 weeks and 50 weeks, respectively. Results In control group A and B, no hyperplasia/dysplasia and carcinoids were ob-served. In Hp-infected groups (C, D, E,), Anti-Hp antibodies and serum gastrin levels significantly in-creased at 50 week,75 weeks and 100 weeks, and the incidences of hyperplasia/dysplasia and carcinoids were 27.8% (5/18), 31.2% (5/16), 58.3% (14/24) and 16.7% (3/18), 31.2% (5/16), 62.5% (15/24), respectively, and significantly higher than each control group(P< 0.01). The areas of carcinoids increased accordingly over the time(P < 0.01). After eradication of Hp in group F at 75 weeks and in group G at 50 weeks, values for anti-Hp IgG titer, gastrin levels decreased gradually. The frequencies of hyperpla-sia/dysplasiaand and carcinoids were 25.0% (4/16), 15.4% (2/13) and 37.5% (6/16), 23.1% (3/13), respectively. The incidences of hyperplasia/dysplasia and carcinoids in earlier Hp-eredicated group G were significantly lower than non-eradicated group E (P < 0.001). The areas of carcinoids also marked re-duced compared with non-eradicated group E(P <0.001). There were significant correlations between anti-Hp antibodies and serum gastrin levels(P <0.005), and between serum gastrin levels and carcinoids(P <0.001). Conclusion These results suggested that Hp infection plays a critical role in development of gas-tric carcinoids and eradication of Hp might be effective in preventing development of Hp-related gastric carci-noids in Mongolian gerbils.